Biologics

Authors: Dugat D, Rochat M, Ritchey J, Payton M.
Journal: VCOT

OBJECTIVES:
To quantitatively describe the intramedullary arterial supply of the adult feline tibia and determine if the arterial supply is significantly different from that of adult small dogs.
METHODS:

Authors: Alves EG, Rezende CM, Serakides R, Pereira Mde M, Rosado IR.
Journal: J Feline Med Surg

The purpose of this study was to evaluate the tissue response to a 70% polyhydroxybutyrate and 30% hydroxyapatite composite in the form of a bone implant, placed intracortically in the distal metaphyseal of the right femur, and subcutaneous implants in cats. Samples of the composite were implanted subcutaneously in the dorsolumbar region and the distal metaphyseal region of the right femur of the animals. The study used 12 neutered adult mixed breed cats, weighing an average of 3.5kg.

Authors: Muir P, Kelly JL, Marvel SJ, Heinrich DA, Schaefer SL, Manley PA, Tewari K, Singh A, Suresh M, Hao Z, Plisch E.
Journal: Vet Surg

Objective: To evaluate lymphocyte populations in stifle synovium and synovial fluid of dogs with degenerative cranial cruciate ligament rupture (CCLR). Study Design: Prospective clinical study. Animals: Dogs (n=25) with stifle arthritis and CCLR, 7 dogs with arthritis associated with cartilage degeneration (osteoarthritis [OA]), and 12 healthy Beagle dogs with intact CCL. Methods: Arthritis was graded radiographically in CCLR dogs. After collection of joint tissues, mononuclear cells were isolated and subsequently analyzed using flow cytometry for expression of CD3, CD4, CD8, and CD21.

Authors: Thomas LA, Bizikova T, Minihan AC.
Journal: Vet Surg

Objective: To characterize the in vitro elution and bioactivity of 2 formulations of antibiotics in a novel, dissolvable, cross-linked dextran polymer matrix: Formulation 1-amikacin and clindamycin (AC); Formulation 2-amikacin, clindamycin, and vancomycin (ACV). Study Design: Prospective, in vitro, experimental study. Methods: Aliquots of the antibiotic impregnated polymer were incubated in PBS buffer for 10 days. PBS was changed every 24 hours and concentrations of the antibiotics eluted into saline were quantified.

Authors: Hunt GB, Wong J, Kuan S.
Journal: JSAP

Objectives: To review the success rates for liposuction of lipomas in dogs, report early complications and medium-term outcomes and formulate recommendations on the most appropriate candidates for liposuction. Methods: Retrospective study of 20 dogs with 76 lipomas diagnosed by cytology, in which dry liposuction was attempted. Case records were reviewed for number and size of the lipomas, efficacy of liposuction, frequency and types of complication and likelihood of recurrence. Results: Liposuction was successful in removing 73 of 76 lipomas (96%).

Authors: Nishida H, Nakayama M, Tanaka H, Kitamura M, Hatoya S, Sugiura K, Suzuki Y, Ide C, Inaba T.
Journal: AJVR

Objective-To evaluate effects of transplantation of bone marrow stromal cells (BMSCs) into the CSF for the treatment of chronic spinal cord injury in dogs that had not responded by 1 month after decompressive surgery. Animals-23 dogs. Procedures-Dogs with paraplegia and loss of nociception in the pelvic limbs for at least 1 month after decompressive surgery were assigned to transplantation or control groups. Dogs in the transplantation group received BMSCs injected into the CSF 1 to 3 months after decompressive surgery. Dogs in the control group did not receive additional treatments.

Authors: Isola M, Ferrari V, Miolo A, Stabile F, Bernardini D, Carnier P, Busetto R.
Journal: VCOT

Objective: To measure the concentrations of nerve growth factor (NGF) in the synovial fluid from normal dogs and dogs with osteoarthritis (OA) secondary to common joint disorders. Methods: Nerve growth factor synovial concentrations were measured by ELISA assay in 50 dogs divided into three groups: 12 healthy, 16 affected by acute lameness within seven days before enrolment, and 22 with chronic lameness persisting by more than one month before enrolment and accompanied by radiological signs of OA.

Authors: Clements DN, Kennedy LJ, Short AD, Barnes A, Ferguson J, Ollier WE.
Journal: VCOT

Objectives: To investigate the association of the major histocompatability (MHC) class II allele haplotype frequencies with the diagnosis of cranial cruciate ligament (CCL) rupture in two breeds of dog. Methods: DNA samples from populations of Labrador Retrievers and Golden Retrievers with CCL rupture and general populations of the same breeds were characterised for three DLA class II loci (DRB1*, DQA1* and DQB1*) alleles using sequence-based typing or reference strand-mediated conformation analysis.

Category: Biologics - Knee
Authors: Arnault F, Maitre P, Cachon T, Carozzo C, Fau D, Genevois JP, Viguier E.
Journal: VCOT

Objective: To report the successful use of recombinant human bone morphogenetic protein-2 (rhBMP-2) in the treatment of an antebrachial non-union, secondary to gunshot fracture, stabilized with circular external fixation in a cat. Procedure: Following a diagnosis of a highly comminuted gunshot fracture of the distal right radius and ulna, the fracture was stabilized with a circular external fixation. A two-ring frame was placed in closed fashion. Results: The skin wound healed uneventfully by second intention in 21 days. Twenty-eight weeks after external fixation, bone healing was absent.

Authors: Verwilghen D, Grulke S, Kampf G.
Journal: Vet Surg

Objective: To assess current habits for surgical hand preparation among veterinary surgical specialists and to compare data with current guidelines for hand asepsis techniques. Study design: Survey of veterinary surgical specialists. Sample Population: Diplomates of the American (ACVS) and European Colleges of Veterinary Surgeons (ECVS). Methods: An internet-based survey of hand preparation methods before surgical procedures was conducted of 1300 listed ACVS and ECVS Diplomates. Results: A 42.6% response rate was obtained.