Biologics

Authors: Medl N, Guerrero TG, Hölzle L, Hässig M, Lochbrunner S, Montavon PM.
Journal: Vet Surg

OBJECTIVE: To (1) determine suction tip (intermittent and continuous mode) contamination rate in orthopedic surgery in dogs and cats; (2) examine the effect of surgical time on contamination; and (3) report bacteria isolated. STUDY DESIGN: Clinical study. SAMPLE POPULATION: Clean orthopedic surgeries (n = 50). METHODS: Surgical procedures were assigned to 1 of 2 groups: (1) continuous (n = 25) or (2) intermittent suction (n = 25). A control suction was operated in each surgery.

Authors: Webb TL, Quimby JM, Dow SW.
Journal: J Feline Med Surg

Mesenchymal stem cells (MSC) are increasingly being proposed as a therapeutic option for a variety of different diseases in human and veterinary medicine. At present, MSC are most often collected from bone marrow (BM) or adipose tissue (AT) and enriched and expanded in vitro before being transferred into recipients. However, little is known regarding the culture characteristics of feline BM-derived (BM-MSC) versus AT-derived MSC (AT-MSC). We compared BM-MSC and AT-MSC from healthy cats with respect to in vitro growth and cell surface phenotype.

Category: Biologics
Authors: Ireifej S, Marino DJ, Loughin CA, Lesser ML, Akerman M.
Journal: Vet Surg

OBJECTIVE:
To report incidence and relevance of positive intraoperative total hip replacement (THR) bacterial cultures taken at the time of surgical closure in dogs having unilateral THR.
STUDY DESIGN:
Retrospective case series.
ANIMALS:
Dogs (n = 100) with cemented (CFX), cementless (BFX), or hybrid THR.
METHODS:

Authors: Little JP, Horn TJ, Marcellin-Little DJ, Harrysson OL, West HA.
Journal: AJVR

Objective-To design and fabricate fiberglass-reinforced composite (FRC) replicas of a canine radius and compare their mechanical properties with those of radii from dog cadavers. Sample-Replicas based on 3 FRC formulations with 33%, 50%, or 60% short-length discontinuous fiberglass by weight (7 replicas/group) and 5 radii from large (> 30-kg) dog cadavers. Procedures-Bones and FRC replicas underwent nondestructive mechanical testing including 4-point bending, axial loading, and torsion and destructive testing to failure during 4-point bending.

Authors: Fitzpatrick N, Smith TJ, Pendegrass CJ, Yeadon R, Ring M, Goodship AE, Blunn GW.
Journal: Vet Surg

OBJECTIVE:
To report clinical application of intraosseous transcutaneous amputation prosthesis (ITAP) for limb salvage.
STUDY DESIGN:
Retrospective case series.
SAMPLE POPULATION:
Client owned dogs with malignant neoplasia of the distal aspect of the limb.
METHOD:

Authors: Kukanich B, Bidgood T, Knesl O.Kukanich B, Bidgood T, Knesl O.
Journal: Vet Anaesth Analg

Objectives   To discuss the clinical pharmacology of currently licensed veterinary NSAIDs and to review gastrointestinal and renal adverse effects as well as drug-drug interactions that have been reported with these drugs. To review the use of NSAIDs in the peri-operative setting and their use in patients with osteoarthritis. To further review the reported effects of NSAIDs on canine articular cartilage and liver as well as the clinical relevance of a washout period.

Authors: Dugat D, Rochat M, Ritchey J, Payton M.
Journal: VCOT

OBJECTIVES:
To quantitatively describe the intramedullary arterial supply of the adult feline tibia and determine if the arterial supply is significantly different from that of adult small dogs.
METHODS:

Authors: Farrell M, Lindsay S, Johnson V, Child G.
Journal: VCOT

An eight-month-old mixed-breed dog was presented with a history of sudden onset pelvic limb lameness. Radiographic and computed tomographic examinations demonstrated an osteolytic process involving the lumbar spine and pelvis. A comprehensive work-up including serial radiographic skeletal survey, biopsy, routine laboratory investigation and evaluation of parathyroid hormone (PTH) and 25-hydroxy-vitamin D levels failed to reveal any underlying cause for the osteolysis.

Authors: Muir P, Kelly JL, Marvel SJ, Heinrich DA, Schaefer SL, Manley PA, Tewari K, Singh A, Suresh M, Hao Z, Plisch E.
Journal: Vet Surg

Objective: To evaluate lymphocyte populations in stifle synovium and synovial fluid of dogs with degenerative cranial cruciate ligament rupture (CCLR). Study Design: Prospective clinical study. Animals: Dogs (n=25) with stifle arthritis and CCLR, 7 dogs with arthritis associated with cartilage degeneration (osteoarthritis [OA]), and 12 healthy Beagle dogs with intact CCL. Methods: Arthritis was graded radiographically in CCLR dogs. After collection of joint tissues, mononuclear cells were isolated and subsequently analyzed using flow cytometry for expression of CD3, CD4, CD8, and CD21.

Authors: Thomas LA, Bizikova T, Minihan AC.
Journal: Vet Surg

Objective: To characterize the in vitro elution and bioactivity of 2 formulations of antibiotics in a novel, dissolvable, cross-linked dextran polymer matrix: Formulation 1-amikacin and clindamycin (AC); Formulation 2-amikacin, clindamycin, and vancomycin (ACV). Study Design: Prospective, in vitro, experimental study. Methods: Aliquots of the antibiotic impregnated polymer were incubated in PBS buffer for 10 days. PBS was changed every 24 hours and concentrations of the antibiotics eluted into saline were quantified.